薄煤层无煤柱开采综合防灭火技术

为了确保晋华宫8209无煤柱工作面在过留巷段期间的安全回采,分析了过留巷段前和过留巷段后采空区漏风与传统开采方式采空区漏风的区别,发现后留巷段后采空区处于一种开放式状态,增加了采空区的漏风量.为此提出了加强预报、喷洒阻化剂、进风顺槽端头封堵、采空区注浆注氮综合防灭火技术,其中进风顺槽端头封堵后采空区漏风量由175 m3/min降低到60 m3/min^90 m3/min,注氮9 d和10 d后5209巷回风流中CO和C2 H6气体浓度分别从0.0014%和0.0628%降到0.该综合防灭火技术有效防止了采空区自燃事故的发生,为无煤柱开采采空区防灭火提供了借鉴.     

A novel joint bidding technique for fuel cell wind turbine photovoltaic storage unit and demand response considering prediction models analysis Effect's

The stake of distributed generation resources like fuel cell in daily market is proved to be a major uncertain problem. The volatile character of market price together with the unbalanced nature of power can take hold of economic advancement of distributed generation resources which in turn can culminate in diversion retribution while the market is being struck. This study introduces a market participation model in share conditions to improve the profit for Fuel Cell/wind turbine/storage/photovoltaic and demand response. To solve the mentioned problem, an accurate prediction model is presented in this paper. This model is based on complete ensemble empirical mode decomposition, and multiple artificial neural network which is coupled with Broyden water cycle algorithm. By this algorithm, the prediction accuracy of proposed forecast engine is enhanced and could get the better results. A sure-footed stochastic optimization approach was deployed in order to take prices of markets and distributed generation resources into account. In the generation of distributed generation resources, forecasting error database in everyday, modified, and depressed market was drawn on to induce probabilistic scenario. Improbable variables were discarded by a neuro-fuzzy model. Eventually, to illustrate the joint model strategy suggested in the study, a testing system contains fuel cell/wind turbine/storage unit/photovoltaic and demand response was utilized and the attained results were calculated in two different periods.     

海参废渣皂苷通过核苷酸结合寡聚化结构域样受体信号通路抵抗环磷酰胺诱导小鼠的免疫抑制

在我国海参是一种具有多种生物活性的传统滋补品。本实验系统性地研究了2.5、5.0、10.0 mg/（kg mb·d）海参废渣皂苷（saponins extracted from sea cucumber residues，SCRSS）对免疫抑制小鼠的调节作用。结果表明，与模型对照组相比，5.0 mg/（kg mb·d）SCRSS使小鼠的体质量、脾脏指数和胸腺指数分别增加了15.3%、23.8%和39.8%；5.0 mg/（kg mb·d）SCRSS也极显著增加了免疫抑制小鼠的吞噬指数（P＜0.01）。此外，与模型对照组相比，各剂量SCRSS均明显地增加了血清中细胞因子的水平，并促进了脾淋巴细胞和腹腔巨噬细胞的增殖活性。其中，中等剂量（5.0 mg/（kg mb·d））SCRSS可以更有效地上调免疫抑制小鼠核苷酸结合寡聚化结构域蛋白1、核苷酸结合寡聚化结构域蛋白2、受体相互作用蛋白-2和核因子-κB的表达。结论：SCRSS具有作为提高免疫力的功能性食品补充剂的潜力。     

一种基于双重ddPCR的肉制品中牛源性成分的定量分析方法

将低价肉类原料掺入高价肉制品是一种典型的肉类掺假方式，为准确鉴别牛肉及牛肉制品的真伪，建立一种基于双重微滴式数字聚合酶链式反应（droplet digital polymerase chain reaction，ddPCR）的牛源性成分定量分析方法。通过对14 种动物的Beta-actin单拷贝基因序列进行分析，设计牛源性特异性引物、探针与动物源性成分通用引物、探针并建立双重ddPCR体系，推导出牛源性成分质量与其特异性扩增拷贝数之间的计算公式，对牛源性成分进行定量检测该方法得到牛源性成分质量M牛（mg）与其特异性扩增拷贝数浓度C牛（copies/μL）之间的计算公式为M牛=0.033C牛＋2.37，对已知牛肉质量的混合肉样品与不同部位的牛肉样品进行检测，结果显示牛肉定量检测值与实际质量基本一致。同时拷贝数相对含量可辅助判断肉制品中是否存在非牛源性的其他动物源性成分掺假。对市售样品的检测发现，存在目标肉样含量不达标以及不同程度的动物源性成分等掺假现象，说明该检测方法可为牛肉制品掺假量化判定和混合源性产品分级鉴定提供技术支撑。     

杏鲍菇富硒蛋白的营养结构特性及对铅毒性的缓解作用

铅主要通过饮食进入人体并在体内积累，对人体免疫和代谢等系统造成损伤，补充硒可以显著改善铅暴露对机体的危害。本研究以杏鲍菇富硒蛋白（selenium-enriched protein from Pleurotus eryngii，SePEP）为原料，通过高效液相色谱-电感耦合等离子体质谱联用技术、傅里叶变换红外光谱等测定蛋白硒形态及结构。通过细胞实验探究蛋白体外模拟消化产物对Pb2＋引起的RAW264.7细胞毒性的缓解作用。结果表明，SePEP硒含量为（360.64±3.11）mg/kg，硒形态主要包括硒代蛋氨酸（SeMet，（48.04±0.64）%（相对含量，下同））、硒代胱氨酸（SeCys2，（31.91±0.51）%）和甲基硒代半胱氨酸（MeSeCys，（14.65±0.36）%）。硒的添加显著促进了氨基酸的生成，改变了蛋白质的结构，α-螺旋结构相对含量由（20.30±0.87）%增加至（25.00±1.60）%，无规卷曲结构相对含量由（20.38±0.84）%减少至（13.85±1.66）%，总巯基与二硫键含量及表面疏水性均显著增加（P＜0.05）。添加75 μg/mL SePEP消化产物后，Pb2＋处理的RAW264.7细胞存活率由接近50%显著升高至（76.95±6.95）%，细胞培养液中乳酸脱氢酶释放量降低57.45%，并且3 种促炎细胞因子白细胞介素（interleukin，IL）-6、IL-8和肿瘤坏死因子α的释放受到显著抑制（P＜0.05），说明SePEP消化产物对Pb2＋暴露引起的RAW264.7细胞损伤有缓解作用。本实验研究成果可为研发安全、有效的改善铅毒性的功能富硒蛋白食品提供参考。     

间隙填充电弧增材制造对表面质量的影响

针对单层多道增材制造,提出一种新的搭接模型——间隙填充模型。研究间隙填充模型对多道焊缝表面质量的影响,发现基体中心间距和接触角对成形件表面质量有重大影响。通过计算平均高度、总熔宽、方差和极差定量比较间隙填充模型和传统搭接模型的表面质量。结果表明:间隙填充模型沉积的多道焊缝表面质量明显优于传统搭接模型,提升了表面质量,节约了材料和时间成本。     

基于时序数据的云网协同平台人工智能运维体系

云计算在企业应用中的拓展不但表现为平台规模的拓展，也表现为平台应用的延伸。“云网协同”和“微服务化”是当前企业云平台演进的重要趋势。随着企业信息化建设重要性的持续提升，微服务化云网协同平台的运行维护面临极大挑战。首先分析了平台运维面临的挑战，梳理了平台人工智能运维需求，提出了基于时序数据分析的平台人工智能运维技术体系，并给出了云网协同平台人工智能运维子系统参考设计。所提技术体系和系统设计具有实用性和推广性，可以作为企业云平台建设和优化的技术途径参考。     

Rebranding Terroir: Ethnoecology and Tea Agro-Ecosystem Management in Response to Increased Market Integration in Southern Yunnan,China

Prompted by increased domestic and transnational demand for Pu’er tea, an emergence of agroecosystem intensification in Southern Yunnan, China has resulted in various agro-ecosystems including tea forests, mixed crop systems, and monocultural terrace tea gardens, in the tea production system. Plants of Camellia sinensis assamica often grow as trees in forests whilst C. sinensis sinensis grow as shrubs in terrace tea gardens. Inspired by the wine industry, the concept „Terroir” acts as a framework that analyzes both environmental and human factors yielding var ying botanic profiles, and hence quantifies values created by the cultivation process. The approach allows economic opportunities of place-based tea products to be driven by the origin in lieu of extrinsic qualities, which has resulted in to the fabricated reputation of terroir. In response to a common gap in terms of botanical and cultural values between tea cultivation and marketing trends, this artic le investigates an alternative scenario in which tea production and promotion model could minimize its environmental impacts and utilize its economic weight to advance land conservation efforts specific to cultural complexity at community scales.     

具有保护肠上皮细胞HT-29免受大肠杆菌和H2O2损伤潜力的植物乳杆菌的筛选

益生菌可在肠道定植从而发挥抗炎或抗氧化活性，有利于宿主肠道健康。本实验研究了从新疆传统发酵乳制品中分离得到的8?株植物乳杆菌对大肠杆菌侵袭和过氧化氢刺激肠上皮细胞HT-29的保护作用。结果表明：在8?株植物乳杆菌中，植物乳杆菌35具有最高的黏附能力。植物乳杆菌35可通过取代、竞争、排阻的方式抑制大肠杆菌对HT-29细胞的黏附，抑制率分别为42.60%、59.17%、60.19%。植物乳杆菌35及其多糖可抑制大肠杆菌刺激HT-29细胞产生白细胞介素-8；同时保护HT-29细胞免受过氧化氢的损伤，增加超氧化物歧化酶、谷胱甘肽过氧化物酶活力水平并降低丙二醛含量。结论：植物乳杆菌35及其粗胞外多糖具有抑制大肠杆菌O157诱导的炎症性肠病的潜力。     

Pharmacotherapeutics and molecular docking studies of alpha-synuclein modulators as promising therapeutics for Parkinson’s disease

Parkinson’s disease (PD) is an age-related neurodegenerative ailment that affects dopamine-producing neurons in a specific area of the brain called the substantia nigra of the ventral midbrain. It is clinically characterized by movement disorder and marked with unusual synaptic protein alpha-synuclein accumulation in the brain. To date, only a few Food and Drug Administration (FDA) approved drugs are available on the market for the treatment of PD. Nonetheless, these drugs show parasympathomimetic related adverse events and remarkably higher toxicity; hence, it is important to find more efficacious molecules to treat PD. In our study, We chosen 22 natural compounds as inhibitors that potentially block the alpha-synuclein clump—the pathological hallmark of PD—and provide new avenues for its treatment. Most of these molecules exhibited good pharmacokinetic behaviors, making them decisively favorable drug candidates to cure PD. Molecular docking studies were performed to investigate the binding interactions between natural compounds and alpha-synuclein as anti-Parkinson drug targets. Among the examined compounds, curcumin and piperine emerged as promising phytochemicals with the highest binding affinity, key residual stable bindings and showed a good inhibitory features. Thus, the present study indicates that curcumin and piperine hold the potential to be developed as treatment options against PD. Experimental validations are needed for insights into their mechanism of action and potential clinical application.